Thermo Fisher, A10460, One Shot™ ccdB Survival™ 2 T1R Competent Cells

One Shot™ ccdB Survival™ 2 T1R Competent Cells One ShotccdB Survival 2 T1RCompetent Cells are suitable for the propagation of plasmids containing theccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors. ccdB Survival 2 T1R cells that are made chemically competent can achieve high transformation efficiency of >1 x 109transformants/μg plasmid DNA. TheccdB Survival 2 T1RE. colistrain is derived from the TOP10 strain and offers the same benefits. Mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) allow cloning of both prokaryotic and eukaryotic genomic DNA that contains methylcytosine and methyladenine. This strain also has thelacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. In addition,recA1 andendA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps. ThetonA (also known asfhuA) mutation inccdB Survival 2 T1Rcells confers resistance to bacteriophages T1 and T5, safeguarding samples against contamination. ccdB Survival 2 T1RCompetent Cells offer:
• >1 x 109transformants/μg efficiency
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Support of high-yield preparation of supercoiled plasmid DNA (theendA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (therecA1 phenotype)Convenient and efficient formatOne ShotccdB Survival 2 T1RE. coliare supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells. Genotype:F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araΔ139 Δ(ara-leu)7697galUgalKrpsL(StrR)endA1nupGfhuA::IS2Find the strain and format that fits your needsWe offer other DH strains inchemically competentandelectrocompetent cellformats to meet your specific needs. MultiShot formatsfor high throughput applications are available. Explorebacterial growthmedia formats. Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: ccdB vector propagation
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 11 x 50 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each