Thermo Fisher, A1113802, Puromycin Dihydrochloride

20 mL Puromycin Dihydrochloride is an aminonucleoside antibiotic produced byStreptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of thepacgene.
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.
2–5.
0 μg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/mL. Gibco™ Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.
2–6.
8) in 10 vials each containing 1 mL.
Other Choices and More InformationWe offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
•Contamination control
•Eukaryotic and bacterial selectionSee recommendations forworking concentrations for selection antibiotics.
Learn more about theuse of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
Cell Type: Eukaryotic Cells, Prokaryotic Cells
Concentration: 10 mg/mL
Culture Type: Mammalian Cell Culture, Insect Cell Culture
For Use With (Application): Eukaryotic Selection⁄Stable Cell Line Generation
Product Line: Gibco™
Quantity: 20 mL
Shelf Life: 12 Months
Shipping Condition: Dry Ice
Form: Liquid
Product Type: Antibiotic
Sterility: Sterile-filtered
With Additives: HEPES
Unit Size: Each