Thermo Fisher, A11155, PichiaPink™ Secretion Signal Set

PichiaPink™ Secretion Signal Set The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed ProteinBy adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastorissecretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequence encodes a different secretion signal. You can test each secretion signal sequence to find the one that works best for your protein. The PichiaPink™ Secretion Signal Set comes with 8 different secretion signals including:α-mating factor pre-sequence fromSaccharomyces cerevisiaeα-amylase signal sequence fromAspergillus nigerGlucoamylase signal sequence fromAspergillus awamoriSerum albumin signal sequence fromHomo sapiensInulinase signal sequence fromKluyveromcyes maxianusInvertase signal sequence fromSaccharomyces cerevisiaeKiller protein signal sequence fromSaccharomyces cerevisiaeLysozyme signal sequence fromGallus gallusEasily Add a Secretion Signal to Your ProteinThe DNA fragments in the PichiaPink™ Secretion Signal Set are designed to be easily inserted in front of your gene when using the pPINK-HC or pPINK-LC vectors. You can try different secretion signal sequences to see which one gives optimal secretion of your protein. Each signal sequence includes the Kozak sequence for theAOX1gene fromPichia pastoristo ensure efficient translation of your protein. The secretion signal sequences come as phosphorylated duplex oligomers in 40 pmol aliquots. Resuspend oligomers in 40 μL TE buffer before use. Why Choose the PichiaPink™ Yeast Expression System?The PichiaPink™ Yeast Expression System is based on the yeastPichia pastoris. Advantages ofPichia pastorisinclude rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years,Pichia pastorishas been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales. For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech. com. References1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]Bacterial or Yeast Strain: PichiaPink™
Cloning Method: Restriction Enzyme/MCS
Description: Cloning into expression vectors
Expression Mechanism: Cell-Based Expression
For Use With (Application): Protein Expression
Product Type: Secretion Signal Set
Quantity: 8 DNA Duplexes Kit
Shipping Condition: Room Temperature
Promoter: AOX1
Species: P. pastoris
Vector: pPINK-LC, pPINK-HC
Unit Size: Each