Thermo Fisher, A1245605, CHO CD EfficientFeed™ B AGT™ Nutrient Supplement

10 L Gibco™ CHO CD EfficientFeed™ B AGT™ nutrient supplement is a single part dry format product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B AGT™ (Advanced Granulation Technology™) nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.
The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available inliquid formatfor small scale use and experimentation.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Achieve performance comparable to our original liquid catalog product
• Ready for larger scale use with convenient packaging options (10 L and 100 L)Which EfficientFeed™ Nutrient Supplement to Choose?Consider theCHO CD Efficient Feed™ Kitwhen:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0Consider CHOCD EfficientFeed™ A supplementwhen:
• You are currently using CD OptiCHO™ as your base cell growth mediumConsider CHOCD EfficientFeed™ B supplementwhen:
• You are currently using CD CHO as your base cell growth mediumConsiderCD EfficientFeed™ C AGT™ supplementwhen:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0Increased TitersCHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including hydrolysates (Figures 2, 3a, & 3b).
Simple Scale-Up and Cost SavingsThe ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ B AGT™ nutrient supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L catalog liquid as a seamless process, as well as allow for cost savings when purchasing production level volumes.
Comparable Performance to Liquid SupplementEqually important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ B (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.
AGT™ Format Ease of UseCHO CD EfficientFeed™ B AGT™ nutrient supplement delivers dry format scale-up convenience as well as simple reconstitution via water addition and a single pH adjustment step (see Product Profile Sheet below for complete instructions).
For research or further manufacturing use. Not for use in diagnostic procedures.
Cell Line: CHO
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Format: Securitainer Capped Vial
Purity or Quality Grade: Cell Culture Grade, Qualified for Further Manufacturing, Research Grade
Quantity: 10 L
Remarks: Chemically Defined Nutrient Feeding Supplement
Serum Level: Serum-Free
Shipping Condition: Wet Ice
Tested For: Solubility
Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Form: AGT
Product Type: Nutrient Supplement
Sterility: Non-sterile
With Additives: Sodium Pyruvate, Sodium Bicarbonate
Without Additives: No Glutamine, No Phenol Red, No Insulin
Unit Size: Each