Thermo Fisher, A13287, GeneArt™ pYES1L Vector with Sapphire™ Technology

GeneArt™ pYES1L Vector with Sapphire™ Technology GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9. 3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with theGeneArt™ High-Order Genetic Assembly System(cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™. Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
•TRP1to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs inE. coli
• Spectinomycin resistance for selection inE. coli
• 6 Convenient and validated restriction sites flanking the cloning regionAntibiotic Resistance Bacterial: Spectinomycin
Cloning Method: Seamless Cloning
For Use With (Application): Cloning
Number of Fragments: Up to 10 Fragments
Product Line: GeneArt™
Quantity: 10 Reactions
Replication Origin: ARS⁄CEN, oriT
Sample Type: DNA
Selection Marker Eukaryotic: TRP1
Shipping Condition: Dry Ice
Size: 110 kb total (vector plus all inserts)
Vector: pYES1L Linearized
Unit Size: Each