Product Description
CSM Media for Mav203 Yeast Cells The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth ofSaccharomyces cerevisaeMaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:
• CSM (without tryptophan): 0. 74 g
• Yeast Nitrogen Base: 6. 66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 gCompatible with All MaV203 ApplicationsThe CSM Media is optimized for theGeneArt™ High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using theGeneArt™ High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, bothlibraryandsub-cloningscale (cat# 11281-011 and 11445-012). Plate Preparation ProtocolFor each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates. Final Product Type: Agar Plates
For Use With (Application): Optimized for use with the GENEART™ High-Order Genetic Assembly System, A13285
Media Type: CSM Media
Preparation Method: Autoclave
Product Line: GeneArt™
Quantity: 2 L
Shipping Condition: Room Temperature
Strain Designations: MaV203
Form: Liquid, Powder
Product Type: Microbiology Media
Sterility: Non-sterile
Target Organism Class: S. cerevisiae
Unit Size: Each
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Collaboration
Tony Tang
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