Thermo Fisher, A1374401, M9 Minimal Salts (2X)

M9 Minimal Salts (2X) M9 Minimal Salts media is a commonly used basic and chemically defined bacterial growth media for cultivation ofE. colistrains. It provides only the necessary nutrients (carbon, nitrogen, phosphorus, sulfur, and metal sources) required for bacterial growth. Our Gibco M9 Minimal Salts (2X) is a ready-to-use concentrated base formulation that can be supplemented with various carbon sources and amino acids to make a complete M9 medium. This medium is preferred in microscopy ofE. coliexperiments as it has a low autofluorescence and absorbance properties. Growth rates decrease at lower or higher osmolarity, and M9 medium has a lower osmolarity (∼0. 25 Osm/L) thanE. colineeds to optimally grow (∼0. 3 Osm/L). This allows for tight control over bacterial growth. M9 Minimal Salts (2X)formulationFormulation per liter:25. 6 g Sodium phosphate (dibasic) heptahydrate6 g Monopotassium phosphate1 g Sodium chloride2 g Ammonium chloride
• A carbon source needs to be added to required concentration
• Additional components might be needed forE. colistrains (e. g. thiamine for DH5α, leucine for DH10B)Primary applications
• Recombinant protein induction inE. coliin extended growth phase (improved protein expression)
• Microscopy or fluorescence measurements experiments using bacteria
• Metabolic activity studies in bacteria with controlled chemical media compositionGibco media bottlesEach media bottle features an angled opening for simplified pipetting. The 1 L Gibco media bottle uses 39% less source material than comparable media bottles on the market. Both the cap and bottle can be recycled as they are made from highly recyclable high-density polyethylene (HDPE) and polyethylene terephthalate (PETE). Find the media format that fits your needsWe offer other ready-to-use media formulations, such asLB Broth,Terrific BrothorS. O. C recovery medium. Explore other media formats forbacterial growth. Media Type: M9 Minimal Salt (2X)
Preparation Method: Ready-to-Use
Quantity: 1 L
Shipping Condition: Room Temperature
Product Type: Liquid Medium
Species: Escherichia coli
Sterility: Sterile
Target Organism Class: Coliforms
Unit Size: Each