Thermo Fisher, A29249, rhLaminin-521

1 mg Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used withEssential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see theEssential 8™ Adaptation Kit.
rhLaminin-521 is:
• Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Efficient—easily reprogram human fibroblasts to pluripotent stem cells
• Physiologically relevant—expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells with no impact to genetic stabilityTrouble-free transitionWhen used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.
Efficient reprogrammingrhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.
Confident expansionrhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.
Easy scale-uprhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
Cell Type: Stem Cells
Culture Type: Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Purity or Quality Grade: hESC-Qualified
Quantity: 1 mg
Shipping Condition: Dry Ice
Source: Xeno-Free
Classification: Xeno-free
Product Type: Extracellular Matrix
pH: 6 to 8
Unit Size: Each