Thermo Fisher, A34715, TaqMan™ Advanced miRNA Human B Card

TaqMan™ Advanced miRNA Human B Card The Applied Biosystems TaqMan Advanced miRNA Human B Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains one micro-fluidic card, enabling accurate quantitation of 384 unique microRNAs in as little as five hours. Four TaqMan Advanced miRNA Assay endogenous controls are included in the card to aid in data normalization. Details about the included assays are available in the Target List file. The assays can detect and quantify the mature form of the miRNA from 1–10 ng of total RNA from tissue.
Note: TheTaqMan Advanced miRNA cDNA Synthesis Kitis required for preparation of the cDNA template that is used with the TaqMan Advanced miRNA assays.
The kit enables the analysis of:
• Multiple miRNAs from a single amplified sample
• Samples that are limited in quantity, including serum, plasma, or other biological fluidsKey features include:
•Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA assays
•Sensitive—detect as few as 60 copies of input miRNA
•Specific—detect only mature miRNA and distinguish between highly homologous miRNAs
•Small sample input—detect and quantify mature miRNA from as little as 1–10 ng of total RNA or 2 μL of plasma or serum
•Versatile—compatible with tissue and biofluids, including serum and plasmaTaqMan Advanced miRNA cards provide all the advantages of TaqMan Advanced miRNA assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan Advanced miRNA assays in parallel. With the TaqMan Advanced miRNA cDNA Synthesis Kit, TaqMan Advanced miRNA cards enable a comprehensive expression profile consistent with miRBase v21, providing the ideal miRNA profiling solution.
TaqMan Advanced miRNA cards are compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex.
Recommended master mix:TaqMan Fast Advanced Master MixTaqMan Advanced miRNA cards require the use of theTaqMan Advanced cDNA Synthesis Kit, which uses a universal RT chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
Format: 384-well microfluidic card
No. of Reactions: 384 reactions
Product Line: TaqMan™
Product Type: Advanced miRNA Human B Card
Quantity: 384 reactions
Research Category: miRNA
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Species: Human
Volume: 2 μL
Detection Method: Primer-probe
For Use With (Application): miRNA analysis
GC-Rich PCR Performance: High
Label or Dye: 2 Color
PCR Method: qPCR
Reaction Speed: Fast or Standard
Unit Size: Each