BRAND / VENDOR: Thermo Fisher

Thermo Fisher, A34716, TaqMan™ Advanced miRNA Human Endogenous Controls Card

CATALOG NUMBER: A34716

Prix régulier$0.99
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Product Description

TaqMan™ Advanced miRNA Human Endogenous Controls Card The Applied Biosystems TaqMan Advanced miRNA Human Endogenous Controls Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains one card than can be used to evaluate 30 potential endogenous control miRNAs in triplicate for up to four samples. Two exogenous (non-human) controls are included for normalization of sample input.
Features of the TaqMan Advanced miRNA Human Endogenous Controls Card include:
•Reproducibility—pre-spotted assays help reduce pipetting error and minimize experimental variability
•Sample savings—requires only 2 μL purified plasma/serum or 1–10 ng total RNA per sample
•Updated content—assay content updated for mirBase v.
22
•Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
•Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA familiesProduct details:Number of cards:1Number of unique miRNAs (not including exogenous controls):30Exogenous controls:cel-miR-39-3p, ath-miR159aCompatible with the following Applied Biosystems qPCR instruments:Viia7, QuantStudio 7 Flex, and QuantStudio 12k FlexRecommended master mix:TaqMan Fast Advanced Master MixNotes
• TaqMan Advanced miRNA cards require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses a miRNA-specific reverse transcription chemistry.
Detection Method: Primer-Probe Detection
For Use With (Application): miRNA Analysis
Label or Dye: 2 Color
No. of Reactions: 384 reactions
Product Line: TaqMan™
Product Type: Endogenous Controls Card
Quantity: 384 reactions
Reaction Speed: Fast or Standard
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Control Type: Endogenous Control
Format: 384-well Microfluidic Card
RNAi Type: miRNA
Species: Human
Unit Size: Each

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