Thermo Fisher, A35380, SYBR™ Green Fast Advanced Cells-to-CT™ Kit

100 Reactions, 55 μL DNase I The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification. SYBR Green Fast Advanced Cells-to-Ct cell lysis reagents are supplied with highest sensitivity Cells-to-Ct Fast Advanced reverse transcription enzyme mix, 2X RT buffer, and best-in-class PowerUp SYBR Green Master mix. The SYBR Green Fast Advanced Cells-to-Ct Kit gives you the sensitivity you need in the fastest workflow available: get gene expressions analysis results from an entire plate of cells in about 1.
5 hours.
Complete—optimized workflow includes cell lysis reagents with gDNA removal, new Fast Advanced RT enzyme mix, buffer, and new PowerUp SYBR Green Fast Advanced Master MixFast—7-minute sample prep, including DNase treatment, at room temperatureEasy—lyse samples in a tube or directly in culture platesRobust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNAEfficient—contains sufficient reagents to generate 1000 real-time PCR results from 100 starting samplesUnique method eliminates the need to isolate RNA from cultured cellsThe Cells-to-Ct method features a unique lysis reagent that lyses cells and protects RNA. DNA removal occurs simultaneously in the 5-minute lysis step to save time and improve ease of use. Lysis is then stopped by the addition of a 'Stop Solution' that irreversibly binds to the lysis reagents.
The SYBR Green Fast Advanced Cells-to-CT Kit contains Cells-to-Ct Fast Advanced reverse transcription (RT) reagents for cDNA synthesis and PowerUp SYBR Green Master Mix for real-time PCR analysis. Primer sets are provided by the user.
Simple 7-minute sample preparationThe SYBR Green Fast Advanced Cells-to-CT Kit is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in lysis solution for five minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C for a short term or -80C for a long term.
Maximize sensitivity of detectionUnlike some competitor kits that limit the amount of lysate in the RT reaction to 10%, the SYBR Green Fast Advanced Cells-to-CT Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 30% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the PowerUP SYBR Green Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.
Reduced sample loss and transfer errorBecause samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.
Proven performance, proven togetherAll components of the SYBR Green Fast Advanced Cells-to-CT Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits, giving you the sensitivity you need in the fastest sample-to-answer workflow available.
Concentration: 2X
DNase: 55 μL DNase I
Fidelity (vs. Taq): 2X
Format: Tube
Green Features: Fewer resources used and less waste, Less hazardous
High-throughput Compatibility: High-throughput Compatible
No. of Reactions: 100 Reactions
Product Line: Cells-to-CT™
Product Type: Fast Advanced Cells-to-Ct Kit
Purification Target: Lysate
Quantity: 100 reactions
Reagent Type: Cell Lysis
Sample Type: Mammalian Cells
Shipping Condition: Dry Ice
Starting Material: 10-100,000 Cells
Sufficient For: 100 Reactions
Detection Method: SYBR
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
PCR Method: 2-step RT-qPCR
Reaction Speed: 7 min.
Unit Size: Each