Thermo Fisher, A35907, Ion AmpliSeq™ Library Kit Plus

96 Reactions The Ion AmpliSeq Library Kit Plus is designed for rapid preparation of amplicon libraries using Ion AmpliSeq panels. Ion AmpliSeq technology enables scalable, multiplex polymerase chain reactions (PCR) from 12- to 24,000-plex in a single well. Next-generation sequencing (NGS) libraries can be prepared using as little as 1 ng of starting material. Features of the Ion AmpliSeq Library Kit Plus include:Scalable and efficient library preparationEasy and robust protocolBarcoded sample multiplexingScalable and efficient library preparationUsing as little as 1 ng of starting material, highly multiplexed PCR amplification of thousands of genomic target regions yields superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. The primers contain proprietary modifications that enable removal of primer sequences during library preparation, thereby resulting in efficient target assessment during sequencing. Easy and robust protocolThe Ion AmpliSeq Library Kit Plus uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The protocol is simple to use and enables high yield and uniformity, reliably and reproducibly. The resulting libraries are ready for downstream template preparation using the automated Ion Chef System (which also provides the option of automated library preparation using the Ion AmpliSeq Kit for Chef DL8 (Cat. No. A29024)) or the Ion OneTouch 2 System followed by sequencing. The intuitive Torrent Suite Software and optional Ion Reporter Software enables automation of data analysis. Barcoded sample multiplexingThe Ion AmpliSeq Library Kit Plus includes reagents for generating amplicons using Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress Barcode Adapters 1-96 kits or IonCode Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost, and to allow for accurate sample-to-sample comparisons. For Use With (Application): Sequencing
For Use With (Equipment): Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
Libraries: Targeted Sequencing Library
No. of Reactions: 96 Reactions
Product Line: Ion AmpliSeq™
Product Type: Library Kit Plus
Quantity: 96 reactions
Sample Type: DNA
Shipping Condition: Approved for shipment on Wet or Dry Ice
Workflow Step: Library Generation
Format: Kit
Sequencing Type: Genome and DNA Sequencing
Unit Size: Each