Thermo Fisher, A58787, Quantifiler™ Trio Automated DNA Quantification Kit

Quantifiler™ Trio Automated DNA Quantification Kit The Applied Biosystems Quantifiler Trio Automated DNA Quantification Kit is a robust solution that empowers forensic laboratories with accurate and efficient DNA analysis. Included is the Quantifiler Automation Enhancer for use with automated liquid handlers to prevent bubbles during pipetting and mixing.
This kit offers simultaneous quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. Here are the key features and benefits of the Quantifiler Trio kit:Highly accurateDetermines the ratio of human male to female DNA, even with minute male and excess female DNA (1:4000 or greater)Includes a pooled, human genomic DNA standard for improved stability and homogeneityHighly sensitive<1 pg/μL limit of detectionQuantifies DNA concentrations from 0.
005 ng/μL to >50 ng/μL, enabling a broad range of concentrationsFlexible protocols for higher concentration samplesHighly specificDetects human and higher primate DNAThree human-specific target loci: small autosomal, large autosomal, and a Y-chromosome targetHighly informativeQuantifies DNA from various sources, including buccal swabs, blood, semen, tissue, and other human DNA sourcesGuides selection of the optimal AmpFLSTR PCR Amplification Kit (autosomal, Y-STR, or miniSTR)Quality Index improves prediction of STR amplification performance and estimation of DNA quantity in degraded samplesEnhanced inhibition detects the presence of PCR inhibitors, suggesting additional purification or the need for a mini STR kitHighly reliableUses gold standard TaqMan real-time PCR technology widely adopted in forensic laboratoriesUtilizes multiple-copy target loci for improved detection sensitivityReal-time PCR has the widest dynamic range for accurate quantification of low-level samplesHighly efficientDual quantification system minimizes sample consumption and streamlines workflowOptimized chemistry for more efficient multiplexing, faster PCR cycle times, and better inhibitor toleranceResults in approximately one hourA highly informative workflow toolImproved performance to match newer STR kits designed for challenging samplesValidated for use with the HID NIMBUS Presto QNA and HID NIMBUS QNA systems for quantification plate setupPackaging features to minimize reagent contamination and accommodate automated platformsCompatible with commonly used extraction technologies for easy integration into current lab workflowsEnables improved and integrated workflow for more effective processing of forensic casework samplesFully automatable with most liquid handling robotic platformsGene Symbol: Large autosomal (LA) human target, Y chromosome, small autosomal (SA) human target
Hot Start: Yes
No. of Reactions: 960 reactions
Passive Reference Dye: Mustang Purple
Product Type: DNA Quantification Kit
Quantity: 960 reactions
Sample Type: Human DNA
Species: Human
Starting Material: DNA
Target Organism Class: Human
Detection Method: Primer-probe
For Use With (Application): DNA Quantitation
Form: Liquid
Label or Dye: FAM, JUN, VIC, ABY
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each