Thermo Fisher, AM1914, RNAqueous™-4PCR Total RNA Isolation Kit

RNAqueous™-4PCR Total RNA Isolation Kit The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.
5 to 75 mg) or from cells (∽100 cells to ∽1 x 107cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
DNA-free RNA for RT-PCRRapid, phenol-free total RNA isolationNovel reagent for rapid and safe removal of DNase and divalent cationsUsing the RNAqueous-4PCR KitThe RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Not high-throughput compatible (Manual)Applications:DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and TissuesElution Volume: 50–120 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 10 min. (After sample homogenization)
Quantity: 30 Preps
Shipping Condition: Box 1: Dry Ice, Box 2: Room Temperature
Starting Material Amount: Tissue: ≤75 mg, Cells: ≤107
Yield: ≤10 μg per 1 mg tissue, ≤1 μg per 1 x 105cells
Isolation Technology: Silica Spin Column
Sample Type: Tissue, Cells
Unit Size: Each