Thermo Fisher, AM1931, RNAqueous™-Micro Total RNA Isolation Kit

RNAqueous™-Micro Total RNA Isolation Kit The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.
g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samplesHow it worksSamples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
Elution Volume: 10–20 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 15 min. (After sample homogenization)
Quantity: 50 Preps
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Starting Material Amount: Cells: ≤105Tissue: ≤10 mg
Isolation Technology: Silica Spin Column
Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM)
Unit Size: Each