Thermo Fisher, AM8439, 5-(3-Aminoallyl)-dUTP (50 mM)

Thermo Fisher, AM8439, 5-(3-Aminoallyl)-dUTP (50 mM) When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this modified nucleotideUse 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.
Concentration: 50 mM
Product Line: Ambion™
Product Type: Nucleotides
Purification Method: HPLC
Quantity: 50 μL
Shipping Condition: Dry Ice
For Use With (Application): Nucleic Acid Labeling
Form: Liquid
Label or Dye: Aminoallyl
Unit Size: Each