Thermo Fisher, AMB13345, MEGAscript™ T7 Transcription Kit

Thermo Fisher, AMB13345, MEGAscript™ T7 Transcription Kit The MEGAscript™ T7 Kit is an ultra-high yieldin vitrotranscription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.
Using the MEGAscript™ T7 KitThe MEGAscript™ T7 Kit containsin vitrotranscription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
ApplicationsThe MEGAscript™ T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, includingin vitrotranslation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Kmfor the enzyme. The MEGAscript™ T7 Kit typically yields over 10 times more RNA than conventionalin vitrotranscription reactions. The kit is not recommended for synthesis of high specific activity probes.
Accessory productsMessageAmp™ aRNA amplification kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purifyin vitrotranscription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).
For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions: 200 Reactions
Product Line: Ambion™, MEGAscript™
Product Type: T7 In Vitro Transcription kit
Promoter: T7
Quantity: 200 reactions
Shipping Condition: Dry Ice
Starting Material: PCR Products, Linearized DNA plasmid
Yield: 100 μg
Final Product Type: Synthetic mRNA, mRNA
Format: Kit
Sample Type: mRNA, Synthetic mRNA
Unit Size: Each