Thermo Fisher, B7277, Bacteria Counting Kit, for flow cytometry

Thermo Fisher, B7277, Bacteria Counting Kit, for flow cytometry The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is also available separately (Cat No. S34855), a high-affinity nucleic acid stain that easily penetrates both gram-negative and gram-positive bacteria, producing an exceptionally bright green-fluorescent signal. The kit also includes a calibrated suspension of polystyrene microspheres. Signals from both the stained bacteria and the beads are easily detected in the green fluorescence channel and can be distinguished on a plot of forward scatter versus fluorescence. The density of the bacteria in the sample can be determined from the ratio of bacterial signals to microsphere signals in the cytogram.
Any physiological buffer between pH 7.
0–8.
0, including PBS, can be used to dilute the SYTO dyes for the staining solution.
View additional information about allmicrobiology assays for flow cytometry.
Detection Method: Fluorescence
Diameter (Metric) Bead: 6 μm
For Use With (Application): Flow Cytometry
For Use With (Equipment): Flow Cytometer
Includes: BC Bacterial Stain, Microsphere Standard
Quantity: 1 kit
Sample Type: Bacteria
Shipping Condition: Room Temperature
Product Type: Bacteria Counting Kit
Unit Size: Each