Thermo Fisher, C10102, Click-IT™ AHA (L-Azidohomoalanine)

Click-IT™ AHA (L-Azidohomoalanine) Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® Protein Analysis Detection Kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein. Detection Method: Biotin-based, Fluorescence
Green Features: Less hazardous
Labeling Method: Metabolic Labeling
Product Line: Click-iT™, Molecular Probes™
Product Type: AHA
Quantity: 5 mg
Shipping Condition: Room Temperature
Labeling Target: Proteins
Label or Dye: Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
Unit Size: Each