Thermo Fisher, C10429, Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit

Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit The Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor™ 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor™ 594 azide leads to a chemoselective ligation or “click' reaction between the green fluorescent azide and the alkyne, allowing the modified proteins to be detected by imaged-based analysis.
•Non-radioactive alternative—an alternative to the traditional35S-methionine
•Visualize bulk protein dynamics—fluorescent tagging of proteins allows their localization to be determined, including aggregation
•Specificity—selective, specific reaction between label and detection tags
•Stability—product contains an irreversible, covalent bond
•Multiplex-enabled—use in conjuction with Click™-iT AHA (azide amino acid and alkyne dye) to detect spatial and temporal differences
•Applicability to biological samples—easy detection; high sensitivity and low background, regardless of complexityThe Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit has been successfully tested in HeLa, A549, and U-2 OS cells with a variety of reagents that inhibit protein synthesis, including cycloheximide and anisomycin. The applicability of these probes to monitor protein degradation has also been shown using inhibitors of the proteasome (MG132 and Bortezomib) and blockers of autophagy (chloroquine) in HeLa cells.
Additionally, due to differences in Click-iT™ chemistry between the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit andClick-iT™ AHA Alexa Fluor™ 488 HCS Kit, these kits can be used in conjunction for spatial or temporal determination of differences in nascent protein synthesis.
Color: Red-Orange
Detection Method: Fluorescence
For Use With (Equipment): Fluorescence Microscope, Fluorescent Imager
Format: Liquid
Green Features: Less hazardous
Label Type: Alexa Fluor Dyes
Product Line: Alexa Fluor™, Click-iT™
Product Type: Protein Synthesis Assay Kit
Quantity: 25 coverslips or two 96-well plates kit
Labeling Target: Proteins
Label or Dye: Alexa Fluor 594
Unit Size: Each