Thermo Fisher, C11765500CP, Ham's F-12 Nutrient Mix Gibco™

Ham's F-12 Nutrient Mix Gibco™ Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.
This Ham's F-12 is modified as follows:With
• L-glutamine
• Phenol RedCompared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). F-12 uses a sodium bicarbonate buffer system (1.
176 g / L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Gibco® 'BASIC' products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.
Unit Size: 10 × 500 mL