Thermo Fisher, E10215, EnzChek™ Direct Phospholipase C Assay Kit

EnzChek™ Direct Phospholipase C Assay Kit This kit provides continuous monitoring of phosphotidyl choline specific phospholipase C (PLC) activity in microplate based biochemical assays. The assay uses a PCL selective fluorogenic substrate that emits a bright green emission upon cleavage.
The EnzChek™ Direct Phospholipase C Assay Kit provides a simple and robust method for monitoring PC-PLC activity. Each kit provides enough reagents for 2 microplates, using 200 μl volumes in 96 well format. PC-PLC plays a crucial role in many cell signaling pathways involved in apoptosis and cell survival, as well as diseases ranging from cancer to HIV1-7. The assay uses a glycerophospho-ethanolamine with a dye-labeled sn-2 acyl chain as a substrate for PC-PLC. Substrate cleavage by PC-PLC before the phosphate releases the dye-labeled diacylglycerol, which produces a positive fluorescence signal that may be measured continuously. The reaction product has absorption and fluorescence emission maxima of 509 nm and 516 nm, respectively. Using purified enzyme from Bacillus cereus, the assay can detect as little as 10 mU⁄mL PC-PLC after one hour at room temperature. The kit has been proven useful for characterizing PC-PLC inhibition, and since it offers a direct measurement, the potential for false positives in a compound screen is eliminated.
Detection Method: Fluorescence
Dye Type: BODIPY™ FL
Filter Type: FITC (Fluorescein) Filter
Quantity: 1 kit
Shipping Condition: Room Temperature
Substrate Properties: Lipid-Based Substrate, Chemical Substrate
Substrate Type: Phospholipase Substrate
Target Enzyme: Phosphatidylcholine-Specific Phospholipase C
Color: Green
Emission: 509/516
For Use With (Application): Phospholipase Assay
For Use With (Equipment): Microplate Reader
Product Line: EnzChek, Molecular Probes
Product Type: Phospholipase Assay
Unit Size: Each