Thermo Fisher, E35350, Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex)

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 555 Streptavidin (Alexa Fluor™ 555 EGF complex) We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green™ 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor™ dyes and Texas Red™ dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.
Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor™ 555 streptavidin (∼555/565 nm)
• Number of fluorophore molecules on each EGF molecule: ∼2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeterFind More Receptor Binding and Phagocytosis ProbesWe offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. ReviewProbes for Following Receptor Binding and Phagocytosis—Section 16.
1in the Molecular Probes™ Handbook for more information on these products.
For Research Use. Not for human or animal therapeutic or diagnostic use.
Cell Type: Mammalian Cells
Detection Method: Fluorescence
Label or Dye: Alexa Fluor Dyes
Product Line: Alexa Fluor™
Quantity: 100 μg
Shipping Condition: Wet Ice
Form: Lyophilized
Product Type: Growth Factor
Unit Size: Each