Thermo Fisher, E6638, EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays

EnzChek™ Protease Assay Kit, green fluorescence, 100-1000 assays The EnzChek™ Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.
See our complete line of Fluorescence Microplate assays.
• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settingsThis EnzChek™ Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY™ FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY™ FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY™ FL dye has excitation and emission spectra similar to those of fluorescein.
Detection Method: Fluorescence Intensity
Quantity: 1 kit
Research Category: Differentiation
Shipping Condition: Room Temperature
Substrate Properties: Protein-Based Substrate
Substrate Type: Protease Substrate
Target Enzyme: Proteases
For Use With (Application): Protease Assay
For Use With (Equipment): Fluorescence Microplate Reader
Product Line: EnzChek
Product Type: Protease Assay
Unit Size: Each