Thermo Fisher, EC0112, DH5α Competent Cells

DH5α Competent Cells Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.
• High transformation efficiency: >1x109cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.
O.
C. Outgrowth medium includedApplicationsDH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesisGenotypeF– φ80lacZΔ M15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+)phoA supE44λ-thi–1 gyrA96 relA1Product Type: Competent Cell
Contains F' Episome: Lacks F' Episome
Efficiency: High Efficiency (>1x10^9 cfu/μg pUC19 DNA)
Cell Type: Chemically Competent
Libraries: cDNA
Cloning Methylated DNA: No
Bacterial or Yeast Strain: DH5α
Blue/White Screening: Yes
High-throughput Compatibility: Not High-throughput Compatible
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Species: E. coli
Format: Tube
Product Line: Thermo Scientific™
For Use With (Application): Cloning
Quantity: 10 x 100 μL
Unit Size: Each