Product Description
Thermo Fisher, EF0221, Pyrophosphatase, inorganic (0.
1 U/μL) Thermo Scientific Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates. The enzyme requires a divalent metal cation with Mg2+conferring the highest activity.
Highlights
• Active in Thermo Scientific buffers forDNA polymerases,RNA polymerases, andReverse TranscriptasesApplications
• High yield RNA synthesis byin vitrotranscription (seeReference 2)
• DNA polymerization reactions: preventing accumulation of pyrophosphate (seeReferences 3, 4)
• Removal of contaminant PPiin reagents used for SNP genotyping by methods based on the detection of pyrophosphateNoteThe enzyme can be diluted with the supplied storage (dilution) buffer. Use of this enzyme in certain applications may be covered by patents and may require a license.
Compatible Buffer: Storage (Dilution) Buffer
Product Type: Pyrophosphatase
Quantity: 10 U
Concentration: 0.
1 U/μL
Enzyme: Pyrophosphatase
Unit Size: Each
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