Thermo Fisher, EN0141, Endonuclease V, T.maritima (5 U/μL)

Thermo Fisher, EN0141, Endonuclease V, T.
maritima (5 U/μL) Thermo Scientific Endonuclease V,T. maritima(Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion.
Highlights
• Optimal activity at temperatures of 65 to 70°CApplications
• High-throughput methods for mutation research
• Studies in mutagenesis and DNA repair
• Mismatch cleavage
• GenotypingNoteUse of this enzyme in certain applications may be covered by patents and may require a license.
When the enzyme is in excess, the primary nicked products experience a second nicking event on the complementary strand, leading to a double-stranded break. At low concentrations, however, Endonuclease V first nicks a DNA strand at the lesions located closer to the 5'-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg2+or Mn2+ions are required for enzyme activity.
Product Type: Endonuclease V
Quantity: 250 units
Concentration: 5 U/μL
Enzyme: Endonuclease
Unit Size: Each