Thermo Fisher, EN0201, RNase H (5 U/μL)

100 units Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications
• Removal of mRNA prior to synthesis of second strand cDNA
•RT-PCRandqRT-PCR: removal of RNA after first strand cDNA synthesis
• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
• Site-specific cleavage of RNA
• Studies ofin vitropolyadenylation reaction productsCompatible Buffer: 10X Reaction Buffer
Product Type: RNase H
Quantity: 100 units
Concentration: 5 U/μL
Enzyme: RNase
Unit Size: Each