Thermo Fisher, EN0531, RNase A, DNase and protease-free (10 mg/mL) 

RNase A, DNase and protease-free (10 mg/mL)  Thermo Scientific RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues.
It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.
HighlightsRNase A is free of DNase activity. It is not necessary to heat it before use.
ApplicationsPlasmid and genomic DNA preparationRemoval of RNA from recombinant protein preparationsRibonuclease protection assays. Used in conjunction with RNase T1Mapping single-base mutations in DNA or RNANotesRecommended concentration of RNase A is 1 to 100 μg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.
3 M or higher, RNase A specifically cleaves single-stranded RNA.
Product Type: RNase A
Quantity: 10 mg
Concentration: 10 mg/mL
Enzyme: RNase
Unit Size: Each