Thermo Fisher, EN0542, RNase T1 (1000 U/μL)

500,000 units Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues. It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.
RNase T1 does not require metal ions for activity.
Applications
• Removal of RNA from DNA preparations
• RNA sequencing
• Ribonuclease protection assays. Used in conjunction withRNase A
• Removal of RNA from recombinant protein preparations
• Determination of the level of RNA transcripts synthesizedin vitrofrom DNA templates containing a 'G-less cassette'Product Type: RNase T1
Quantity: 500,000 units
Concentration: 1000 U/μL
Enzyme: RNase
Unit Size: Each