Thermo Fisher, EO0492, Proteinase K, recombinant, PCR grade

Thermo Fisher, EO0492, Proteinase K, recombinant, PCR grade Thermo Scientific Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents. Proteinase K cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.
Highlights
• Ready-to-use solution
• Active in a wide range of reaction conditionsApplications
• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines
• Determination of enzyme localization
• Improving cloning efficiency of PCR productsNote
• The recommended working concentration of Proteinase K is 0.
05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.
2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase K against autolysis, increases the thermal stability, and has a regulatory function for the substrate binding site of Proteinase K
• Stable over a wide pH range: 4.
0 to 12.
5, optimum pH 7.
5 to 8.
0
• Optimum activity at 50 to55°C
• Rapid denaturation of enzyme occurs at temperatures above 65°CProduct Type: Proteinase K
Quantity: 5 x 1.
0 mL
Concentration: 600 to 1000 U/mL, 14 to 22 mg/mL
Form: Liquid
Unit Size: Each