Thermo Fisher, EO0831, T4 beta-glucosyltransferase

Thermo Fisher, EO0831, T4 beta-glucosyltransferase Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.
Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 μg DNA at 37°C in 15 minutes.
Highlights
•Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
•Fast—complete glucosylation of 1 μg DNA in just 15 min
•Convenient—supplied with optimized buffer and UDP-glucose.
Applications
• Locus specific detection of 5-hmC
• Enrichment of 5-hmC containing DNA
• Labeling of 5-hmC residues using a radioactive UDP-glucose donorIncludes
• T4 β-glucosyltransferase, 5 U/μL
• 10X Epi buffer
• 10X UDP-glucoseCompatible Buffer: 10X Epi Buffer
Product Type: T4 Beta Glucosyltransferase
Quantity: 500 units
Enzyme: T4 β-glucosyltransferase
Unit Size: Each