Thermo Fisher, EP0131, SP6 RNA Polymerase (20 U/μL)

SP6 RNA Polymerase (20 U/μL) Thermo Scientific Bacteriophage SP6 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter and incorporates modified nucleotides.
Highlights
•Incorporates modified nucleotides(e.
g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)Applications
• Synthesis of unlabeled and labeled RNA that can be used:
• For hybridization,in vitroRNA translation
• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• In studies of RNA secondary structure and RNA-protein interactions, RNA splicingConsensus promoter sequence:SP6: ATTTAGGTGACACTATAGAAGNGConcentration: 1X
Quantity: 2,000 units
Polymerase: SP6 RNA Polymerase
Unit Size: Each