Thermo Fisher, F10471, Fluo-4 Direct™ Calcium Assay Kit

100 ml The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:1. Easily loads into cells2. Achieves a large assay window and3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescenceThis advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
Detection Method: Fluorescence
Dye Type: Fluorescent Dye-Based
Form: Powder
Modification: Chemical
Quantity: 10 x 10 mL
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus, Organelles, Cytoplasm
Color: Green
Emission: Visible
For Use With (Application): Calcium Assay
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Line: Molecular Probes
Product Type: Cell-based calcium flux
Unit Size: Each