Thermo Fisher, F14221, Fluo-5F, Pentapotassium Salt, cell impermeant

Fluo-5F, Pentapotassium Salt, cell impermeant Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. Fluo-5F, fluo-5N, and fluo-4ff are analogs of fluo-4 with lower Ca2+-binding affinity, making them suitable for detecting intracellular calcium levels in the 1 μM to 1 mM range that would saturate the response of fluo-3 and fluo-4. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection or ourInflux™ pinocytotic cell-loading reagent. These indicators are compatible with excitation at 488 nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›Calcium Indicator (Cell-Impermeant Salts) Specifications:
• Label (Ex/Em of Ca2+–bound form): Fluo-5F (494/516 nm)
• Fluorescence intensity increase upon binding Ca2+: >100 fold
• Kdfor Ca2+in buffer: ∼2.
3 μM
• Exhibit fluorescence increase upon binding Ca2+with little shift in wavelengthUsing TPEN to Control Heavy Metal CationsIn addition, BAPTA-based indicators such as these bind various heavy metal cations (e.
g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelatorTPEN.
More Choices for Fluorescent Calcium IndicatorsWe offer a large selection of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, reviewFluorescent Ca2+Indicators Excited with Visible Light—Section 19.
3in the Molecular Probes™ Handbook.
For UV-excitable Ca2+indicators, protein-based Ca2+indicators, conjugates of Ca2+indicators, and for fluorescence-based indicators of other metal ions (i.
e., Mg2+, Zn2+) reviewIndicators for Ca2+, Mg2+, Zn2+and Other Metal Ions—Chapter 19in the Molecular Probes™ Handbook.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Detection Method: Fluorescence
Dye Type: Fluorescent Dye-Based
Quantity: 500 μg
Shipping Condition: Room Temperature
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Type: Calcium Indicator
Unit Size: Each