Thermo Fisher, F34953, FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit TheFungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kitcombines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
Cell Type: Yeast Cells
Detection Method: Fluorescence
Dye Type: Other Label(s) or Dye(s)
Form: Solid
Format: Tube(s), Slide(s)
Quantity: 200 kit
Shipping Condition: Room Temperature
Solubility: DMSO (Dimethylsulfoxide)
Emission: PI: 490/635, CFDA: 492/517
For Use With (Application): Viability Assay
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Product Line: FungaLight
Product Type: AM/Propidium Iodide Vitality Kit
Unit Size: Each