Thermo Fisher, F541, Phusion Site-Directed Mutagenesis Kit

Phusion Site-Directed Mutagenesis Kit Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competentE. colicells.
The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For optimal results start by accurately calculating your Tm with ourTm calculator.
Highlights
• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
•Phusion Hot Start II High Fidelity DNA Polymeraseminimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competentE. colicellsFidelity (vs. Taq): 52X
Format: Kit
Hot Start: Yes
Mutagenesis Capability: Single-Site Mutagenesis
No. of Reactions: 20 Reactions
Polymerase: Phusion Hot Start II High Fidelity DNA Polymerase
Product Line: Phusion
Product Type: Site-Directed Mutagenesis Kit
Quantity: 20 reactions
Starting Material: DNA
Form: Solution
GC-Rich PCR Performance: High
Reaction Speed: Fast
Unit Size: Each