Thermo Fisher, F8887, FluoSpheres™ Size Kit #1, Carboxylate-modified Microspheres, red fluorescent (580/605), 2% solids, six sizes

FluoSpheres™ Size Kit #1, Carboxylate-modified Microspheres, red fluorescent (580/605), 2% solids, six sizes Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres™ Size Kit supplies 1 mL of each the 0.
02, 0.
1, 0.
2, 0.
5, 1.
0, and 2.
0 μm sizes.
FluoSpheres™ Microsphere SpecificationsLabel (Ex/Em):Red fluorescent (580/605)Nominal bead diameter:0.
02, 0.
1, 0.
2, 0.
5, 1.
0, 2.
0 μmCoupling surface:CarboxylateSolids:2%Characteristics of the Various FluoSpheres™ Coupling SurfacesCarboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
Aldehyde—sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.
Key Applications of MicrospheresInstrument calibration (flow cytometry, microscopy, HTS, HCS)Flow testing (microfluidics, blood flow, water flow, and air flow)Cell biology tracers (cell differentiation and cell tracing)Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)Choices for FluoSpheres™ Fluorescent MicrospheresAmong our complete offering of fluorescent microspheres products, you'll find beads with these variations:Ten fluorescent colorsTen nominal bead diameters: 0.
02 μm, 0.
04 μm, 0.
1 μm, 0.
2 μm, 0.
5 μm, 1.
0 μm, 2.
0 μm, 4.
0 μm, 10.
0 μm, and 15.
0 μmFour surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amineMicrospheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinumChoices for Unstained MicrospheresWe also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.
We'll Make a Custom Microsphere Product for YouWe will prepare custom orders upon request. For example, FluoSpheres™ beads can be prepared with intensities that arelowerthan those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.
Product Line: FLUOSPHERES™
Quantity: 6 x 1 mL (1 mL/size)
Shipping Condition: Room Temperature
Surface Modification: Carboxylate
Color: Red
Diameter (Metric): 0.
02, 0.
1, 0.
2, 0.
5, 1, 2 μm
Material: Polystyrene
Product Type: Carboxylate-Modified Microsphere
Unit Size: Each