Thermo Fisher, FD0664, FastDigest SmaI

200 μL (200 Reactions) 5' CCC ↓GGG 3'3' GGG ↑CCC 5'Thermo Scientific FastDigest SmaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps.
This restriction enzyme recognizes CCC^GGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: TspMI, XmaCI, XmaI.
DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features100% activity of all FastDigest enzymes in the universal buffer100% buffer compatibility with downstream applicationsComplete digestion in 5–15 minutesDirect loading on gelsNo star activity176 FastDigest enzymes availableApplicationsMolecular cloningRestriction site mappingGenotypingSouthern BlotRestriction fragment length polymorphism (RFLP)SNP analysisNotesThe FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer.
For applications that require product analysis by fluorescence excitation (e.
g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.
For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature: 65°C
Overhang: Blunt
Product Type: Restriction Enzyme
Quantity: 200 μL (200 Reactions)
Enzyme: Sma I
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature: 37°C
Product Line: FastDigest™
Research Category: Traditional Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Unit Size: Each