Thermo Fisher, FD1744, FastDigest BveI

Thermo Fisher, FD1744, FastDigest BveI 5'ACCTGCN4↓3'3'TGGACGN8↑5'Thermo Scientific FastDigest BveI restriction enzyme recognizes ACCTGC(4/8)^ site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BspMI, Acc36I, BfuAI.
Thermo Scientific FastDigest BveI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes availableApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysisNote:At least two copies of FastDigest BveI recognition site are required for efficient cleavage. Inclusion of 0.
5 μM oligonucleotide with the FastDigest BveI recognition sequence in the reaction mixture significantly improves cleavage of plasmid DNAs, especially of those with a single FastDigest BveI site. Still, a complete cleavage of some substrates by FastDigest BveI is difficult to achieve.
The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.
g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.
For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature: 65°C
Overhang: 5' protruding ends
Product Type: Restriction Enzyme
Quantity: 50 μL (50 Reactions)
Enzyme: BveI
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature: 37°C
Product Line: FastDigest™
Research Category: Scarless Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Unit Size: Each