Thermo Fisher, I35102, Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy

Red, Green, Blue, Caspase 3/7, SR-DEVD-FMK, SYTOX Green, Hoechst 33342 Detect and evaluate caspase activation, membrane permeability, and cell cycle using Image-iT LIVE red and green poly and caspase-3 and-7 apoptosis detection kits for microscopy, including fluorescence microscopy and high content screening (HCS). The Image-iT LIVE red and green caspase detection kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity, a measure of cell apoptosis. Specifically, the Image-iT LIVE red and green caspase detection kits contain the substrates SR-VAD-FMK and SR-DEVD-FMK (for red fluorescence), or FAM-VAD-FMK and FAM-DEVD-FMK (green fluorescence), for poly caspases and caspase-3/7 detection, respectively. They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by microscopy and HCS.
Color: Red, Green, Blue
Description: Image-iT LIVE Red Caspase-3 and -7 Detection Kit
Enzyme: Caspase 3/7
Excitation/Emission: SR-DEVD-FMK: 550/595SYTOX Green: 504/523Hoechst 33342: 350/461
For Use With (Equipment): Fluorescence Microscope
Label Type: Other Label(s) or Dye(s)
Label or Dye: SR-DEVD-FMK, SYTOX Green, Hoechst 33342
No. of Reactions: 25 tests (labeling volumes of 300μL)
Product Line: Image-iT
Product Type: Caspase 3/7 Reagent
Quantity: 1 kit
Shipping Condition: Room Temperature
Storage Requirements: Store in refrigerator (2–8°C) and protect from light.
Detection Method: Fluorescence
Format: Slide
Unit Size: Each