Thermo Fisher, K0394, Luminaris Color HiGreen qPCR Master Mix

5000 Reactions **********************Alternative Product:TryPowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.**********************Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:—Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers—Sensitivity: single copy detection—Wide linear range: accurate quantification across 9 orders of magnitude—Reproducibility and convenience: ready-to-use 2X master mixApplications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detectionIncludesLuminaris Color HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
Concentration: 2X
Fidelity (vs. Taq): 2 X
For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
No. of Reactions: 5000 Reactions
Polymerase: Taq DNA Polymerase
Product Line: Luminaris
Product Type: qPCR Master Mix
Quantity: 5,000 reactions
Sample Type: DNA, dsDNA
Sufficient For: 5000 Reactions
Detection Method: SYBR
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each