Thermo Fisher, K155003, RiboMinus™ Transcriptome Isolation Kit, yeast

RiboMinus™ Transcriptome Isolation Kit, yeast RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:
• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
Isolation Technology: Magnetic Bead
Purification Time: 1.
25 hr.
Sample Type: Total RNA (Yeast)
Final Product Type: Transcriptome RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
No. of Reactions: 12 reactions
Product Line: RiboMinus™
Quantity: 12 reactions
Starting Material Amount: Up to 10 μg total RNA
Target: Transcriptome RNA
System Type: RiboMinus
Yield: 1 μg
Form: Magnetic Bead
Unit Size: Each