Thermo Fisher, K157001, PureLink™ miRNA Isolation Kit

PureLink™ miRNA Isolation Kit The Ambion™ PureLink™ miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. The kit provides a rapid and convenient method for purifying small RNA molecules from animal and plant cells, as well as from bacteria and yeast samples.
• Purify small RNA molecules with a novel enrich/bind/elute procedure
• Prepare enriched microRNA samples typically in less than 15 minutes
• Minimize contaminating large RNA molecules and genomic DNARapid and Convenient Column Purification Procedure for small RNAsThe Ambion™ PureLink™ miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes (see Table 1). The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs (see Figure 1). RNAs greater than approximately 200 nucleotides are bound to the first column. Ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the glass fiber membrane in the second column. Once bound to the second column, small RNA molecules are then eluted, ready-for-use, and free of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA, allowing for focused expression analysis studies of small RNA molecules.
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:The miRNA purified using the Ambion™ PureLink™ miRNA Isolation Kit is suitable for downstream applications including RNA sequencing, microarray analysis,real-time PCR (qPCR), reverse transcription real-time PCR (RT-qPCR), andnorthern blotting.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Elution Volume: 50–100 μL
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, Microarray Analysis, Northern Blotting
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 20 min. (After sample homogenization)
Quantity: 25 preps
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤106cellsCells: 106Plant: variesTissue: ≤100 mgYeast: 1 mLDicing Reaction: 300 μL
Yield: Up to 6 μg
Isolation Technology: Silica Spin Column
Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction
Unit Size: Each