Thermo Fisher, K182002, PureLink™ Genomic DNA Mini Kit

250 Preps The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:
•Kit flexibility—one kit works with a variety of sample types and sizes
•Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
•Improved design—optimized spin-column design and buffer formulation for better yield and purityMultiple sample sources, one kitThe PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.
Optimized components and protocolsThe PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.
Higher yields and purityWith PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.
9 for both A260/A280and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).
Elution Volume: 25 to 200 μL
Final Product Type: gDNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Sample Type: Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤109cellsCells: ≤106Tissue: ≤25 mgMouse Tail: 1 cmRat Tail: 0.
5 cmWhole Blood: ≤1 mLYeast: ≤107cellsFFPE: ≤8 sections of 5-15 μM thicknessOragene™ Preserved Saliva: ≤4 mLBlood Spots: ≤5 punches
Test Time: Up to 15 min. after cell lysis
Yield: ≤40 μg
Isolation Technology: Silica Spin Column
Unit Size: Each