Thermo Fisher, K182104A, PureLink™ Pro 96 Genomic DNA Purification Kit

PureLink™ Pro 96 Genomic DNA Purification Kit The PureLink™Pro96 Genomic DNA Purification Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types in a high-throughput platform. This single kit allows genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a familiar silica semi-skirted 96-well plate format. Advantages of the PureLink™Pro96 Genomic DNA Purification Kit:
•Kit flexibility—one kit works with a variety of sample types and sizes
•Ultraclean gDNA—excellent A260/A280and A260/A230absorbance ratios, regardless of sample source
•Improved design—optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifoldsMultiple sample sources, one kitThe PureLink™Pro96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink™ProGenomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.
Improved plate designThe PureLink™Pro96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates (see figure), for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at ∼2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.
Higher yields and purityThe PureLink™Pro96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (see figure). You are also able to get greater yields of higher-purity gDNA (determined by A260/A280measurements) no matter what your sample type is; from bacteria to tissues to blood to cells (see figures). The enhanced PureLink™ Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.
8 for both A260/A280and A260/A230, indicating no contamination carryover of protein or guanidine (see figure).
Elution Volume: 50 to 200 μL
Final Product Type: gDNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
For Use With (Equipment): Biomek™ FX, Eppendorf epMotion™, TECAN Freedom Evo™, TECAN Genesis™, Robotic Liquid Handling Systems
High-throughput Compatibility: High-throughput Compatible
Quantity: 4 x 96 Preps
Sample Type: Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤109cellsBuccal Swab: 1Cells: ≤106Dried Blood Spots: ≤5 punchesFFPE: ≤8 sections of 5-15 μM thicknessMouse Tail: 1 cmRat Tail: 0.
5 cmSaliva (Oragene™ preserved): ≤200 μLTissue: ≤25 mgWhole Blood: ≤200 μLYeast: ≤107cells
Test Time: 45 min. (Including sample homogenization)
Yield: 0.
5 mg (Binding capacity)
Isolation Technology: Silica Plate
Unit Size: Each