Thermo Fisher, K250020, pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli Invitrogen™

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli Invitrogen™ The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. With our patentedattsites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time. The pCR™8/GW/TOPO™ TA Cloning Kit offers:
•attsites for rapid recombination into a variety of Gateway™ destination vectors
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection inE. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of insertsThis kit includes One Shot™ TOP10 Chemically CompetentE. coli. Cloning Method: TOPO™ with Gateway™
No. of Reactions: 20 reactions
Product Line: One Shot™
Product Type: TA Cloning Kit
Quantity: 20 Reactions
Vector: pCR
Unit Size: Each