Thermo Fisher, K278001, Calcium Phosphate Transfection Kit

Calcium Phosphate Transfection Kit The Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation. How it worksThe calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate. Calcium phosphate facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis. The method was first developed by Graham and van der Ebb and was later modified by Wigler. The procedure is routinely used to transfect a wide variety of cell types for transient expression or for producing stable transformants. The DNA is mixed directly with a concentrated solution of CaCl2, which is then added dropwise to a phosphate buffer to form a fine precipitate. Aeration of the phosphate buffer while adding the DNA-CaCl2solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently. For Use With (Application): Transfection
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Type: Transfection Kit
Quantity: 75 Reactions
Serum Compatible: No
Cell Type: Established Cell Lines
Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type: Plasmid DNA
Transfection Technique: Chemical Transfection
Unit Size: Each