Thermo Fisher, K480040, pcDNA™3.1/V5-His TOPO™ TA Expression Kit

40 Reactions, TOPO-TA Vectors, pcDNA The pcDNA™3.
1/V5-His TOPO™ TA Expression Kit offers one-step cloning ofTaq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.
1/V5-His-TOPO™ vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.
In addition, the vector includes the following features:
• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Constitutive Expression
Product Type: TOPO TA Expression Kit
Quantity: 40 Reactions
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: TOPO-TA Vectors, pcDNA
Cloning Method: TOPO-TA
Product Line: TOPO™, pcDNA™
Promoter: CMV
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each