Thermo Fisher, LC6090, Lumio™ Green Detection Kit

Lumio™ Green Detection Kit The Lumio™ Green Detection Kit is a sensitive and highly specific kit for labeling Lumio™ fusion proteins prior to electrophoresis. The Lumio™ Green Kit enables immediate visualization of Lumio™ fusion protein bands directly in a polyacrylamide gel. Using the Lumio™ Green Detection Kit you'll:
• Label proteins fused to a Lumio™ sequence
• Visualize results immediately after electrophoresis—eliminate gel staining and western blotting
• Detect nanogram levels of protein with a UV transilluminator, equipped with a standard camera (Figure 1), or a laser-based scannerThe Lumio™ Green Detection Reagent, included in the Lumio™ Green Detection Kit, can also be used for real-time detection of protein synthesis duringin vitrotranscription-translation reactions.
Immediate and Effortless DetectionTo detect using the Lumio™ Green Detection Kit simply:1. Add the Lumio™ Green Detection Reagent and the optimized Lumio™ Sample Buffer to your protein sample and heat for 10 minutes at 70°C.
2. Add the Lumio™ In-Gel Detection Enhancer to your sample mix and incubate for 5 minutes at room temperature.
3. Load and electrophorese your protein gel.
4. Visualize your Lumio™ fusion protein bands with a UV transilluminator, equipped with a standard camera, or laser-based scanner.
5. After documenting your Lumio™ Green signal, total protein staining can be performed.
Detection Location: In-Gel Detection
Detection Method: Fluorescence
Product Line: Lumio™
Product Type: Pre-Gel Fusion Protein Labeling Kit
Quantity: 1 kit
Shelf Life: 6 Months
Shipping Condition: Dry Ice
Target Molecule: Proteins (Lumio™-Tagged) , Proteins (TC-Tagged)
Label or Dye: Lumio Green
Unit Size: Each